The RNA-binding protein Mex3B regulates airway remodeling through modulation of TGFBR3 mRNA stability in chronic rhinosinusitis.
نویسندگان
چکیده
Abstract Objective: To explore the pathogenic function of Mex3 RNA binding family member B (Mex3b) in chronic rhinosinusitis(CRS). Methods: The expression Mex3b, TGF-β receptor III (TGFBR3), TGF-β2, p-Smad2 were studied by quantitative RT-PCR, immunohistochemistry, western blotting, and ELISA. MEX3b with TGFBR3was test improved immunoprecipitation–coupled high-throughput sequencing (iRIP-Seq). Results: Compared to control subjects, mNRA protein Mex3b nasal epithelial cells from CRSwNP patients are upregulated. And mRNA levels cultured human cells(HNECs) obtained subjects upregulated LPS, CpG IL-1β. Incresed downregulates TGFBR3expression airway TGFBR3mRNA, which results downregulated level TGFBR3in CRS compared subjects. Loss TGFBR3 inhibits TGF-β2 induced gene transcription cells. suppresses signaling activation downregulating correlated tissue edema CRSwNP. Conclusions: upregulation cells, thefore regulates remodeling CRS.
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ژورنال
عنوان ژورنال: Journal of Immunology
سال: 2023
ISSN: ['1550-6606', '0022-1767']
DOI: https://doi.org/10.4049/jimmunol.210.supp.67.24